Figure 3.

Tail-flick test to determine sensory perception to warm stimuli. (A) Heterozygous Btbd9 mutant mice and homozygous Btbd9 mutant mice showed a dramatic decrease in latency to respond to a warm stimulus. (B) Heterozygous Btbd9 mutant mice showed no sensory alteration during the middle of the active phase (midnight), but showed a sensory alteration during the middle of the rest phase (midday). However, after a 0.1 mg/kg injection of ropinirole, a DA receptor agonist, there was no statistical difference in latency to respond 15, 30 or 60 min PI. Top schematic describes the experimental design and setup. Bars represent means with standard errors of the mean. *P < 0.05, **P < 0.01.