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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 13;189(2):956–967. doi: 10.4049/jimmunol.1102871

Figure 7. CCRL2 upregulation on mouse liver endothelial cells following LPS injection in vivo.

Figure 7

Wild-type or CCRL2−/− mice were injected i.p. with LPS. Endothelial cells from the liver were isolated 12 hours later, enriched and identified by co-staining for CD31 and CD146. Surface expression of CCRL2 on these cells was determined using anti-mCCRL2 and Fc-Chemerin (A). VCAM-1 expression was determined by flow cytometry (B). The data set shown is representative of 3–6 individual experiments with similar results. (C) Induction of CCRL2, Fc-Chemerin and VCAM-1 in LPS treated mice was quantified by normalizing the specific antibody MFI value to its isotype MFI value. The mean ± SEM is shown. Each graph represents 3–6 independent experiments pooled together (n=1–2 mice per group per experiment).*p<0.05 by t-test.