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. Author manuscript; available in PMC: 2012 Aug 27.
Published in final edited form as: Comp Immunol Microbiol Infect Dis. 2008 Aug 8;32(2):47–60. doi: 10.1016/j.cimid.2008.05.003

Figure 1.

Figure 1

Map of pMMK-2, which contains both the donor and helper elements of the piggyBac transposon. The donor element (the portion which is integrated) includes the CAG promoter driving the EGFP transgene which is flanked by the 5′-Right terminal and 3′-Left terminal repeats. The piggyBac transposase gene also driven by its own CAG promoter is outside the terminal repeats. The region outside the terminal repeats, including the transposase source is not inserted into the genome of the host and is eventually degraded.