Figure 5.

Transfection efficiencies of LC lipopolyplexes (A), HC lipopolyplexes (B), and AC lipopolyplexes (C), and the corresponding lipoplexes and polyplexes in bone marrow stem cells.

Notes: Complexes were prepared at the indicated PEI/DNA and lipid/DNA ratios. Bone marrow stem cells were incubated for 4 hours with complexes in medium containing 10% fetal bovine serum. Forty-eight hours after transfection, the cells were lysed for quantification of luciferase activity. Data are shown as the mean ± standard deviation (n = 3, *P < 0.05 versus polyplex with a PEI/DNA ratio of 10; ^#P < 0.05 versus HC lipoplex with the lipid/DNA ratio of 2.7).

Abbreviations: LC, lysinylated cholesterol; HC, histidylated cholesterol; AC, arginylated cholesterol; L, lipid; P, polyethylenimine; D, DNA.