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. 2012 Aug 22;7:4637–4648. doi: 10.2147/IJN.S33923

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© 2012 Song et al, publisher and licensee Dove Medical Press Ltd.

This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

PMC Copyright notice

Representative confocal laser scanning microscopy fluorescence images of subcellular localization of various complexes.

Notes: Fluorescein isothiocyanate-labeled PEI is shown in green, rhodamine-labeled lipids are shown in purple, TOTO-labeled DNA is shown in red, and Hoechst-stained nuclei are shown in blue. Bone marrow stem cells were treated for 4 hours with the various complexes formed at the indicated PEI/DNA and lipid/DNA ratios in medium containing 10% fetal bovine serum and observed under confocal laser scanning microscopy (white arrows, free DNA; scale bar 25 μm).

Abbreviations: LC, lysinylated cholesterol; HC, histidylated cholesterol; AC, arginylated cholesterol; L, lipid; P, polyethylenimine; D, DNA.