Figure 9.

hVEGF₁₆₅ mRNA (A) and protein (B) expression by transfected bone marrow stem cells.

Notes: Bone marrow stem cells were transfected with complexes prepared at the indicated lipid/DNA and PEI/DNA ratios in the presence of 10% fetal bovine serum. Cells and the culture supernatant were harvested 48 hours after transfection. hVEGF₁₆₅ mRNA levels in bone marrow stem cells were quantified by real-time polymerase chain reaction. Secretion of vascular endothelial growth factor protein in the culture supernatant was quantified by enzyme-linked immunosorbent assay. Results are normalized for the number of cells and duration of culture, and are expressed as the mean ± standard deviation (n = 3; *P < 0.05 versus the polyplex).

Abbreviations: LC, lysinylated cholesterol; HC, histidylated cholesterol; L, lipid; P, polyethylenimine; D, DNA.