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. 2012 Aug 27;7(8):e43723. doi: 10.1371/journal.pone.0043723

Figure 2. p26 dsRNA specifically knocks down p26 mRNA and protein in Artemia cysts.

Figure 2

A. PCR amplification of p26 mRNA in cysts released by females injected with either control solution (1) or p26 dsRNA (2). Amplification products were resolved in 1.2% agarose gels and stained with Sybersafe. Protein from 25 cysts (B) and 40 cysts (C, D) produced by females injected with either control solution (1) or p26 dsRNA (2) were resolved in 12.5% SDS polyacrylamide gels and blotted to nitrocellulose. The blots were probed with antibody specific to p26 (B), ArHsp21 (C) and ArHsp22 (D), followed by HRP-conjugated goat anti-rabbit IgG antibody. Antibody reactive proteins were visualized by chemiluminescence.