Figure 5. Artemia cysts lacking p26 terminate diapause spontaneously.

A. Cysts lacking (−p26) and containing (+p26) p26 were incubated without agitation in sealed tubes for at least 90 days, transferred to weigh boats and observed with a dissecting microscope until 5 days after the last cyst hatched. The experiment was done in duplicate with different broods of cysts and the error bar represents standard error. B–F. Light micrographs showing the development, or absence thereof, for cysts lacking (B–D) and containing (E, F) p26. c, cyst; e, emerged cyst; es, eye spot. The nauplii shown in Fig. D originated from commercial cysts and they are representative of nauplii obtained from laboratory reared cysts lacking p26.