Figure 7. p26 increases cyst tolerance to heat shock.

A. Hydrated commercial cysts containing p26 were heat shocked for 30 min at the temperatures indicated in the graph, cooled on ice, incubated in sea water at room temperature and observed for hatching. The experiment was done in triplicate and standard error is shown for each measurement. B. Cysts containing (+p26) and lacking (−p26) p26 were either held at room temperature (a) or heated at 41°C for 30 min (b), cooled on ice, incubated in sea water and observed for hatching. Results are presented as the hatching percentage with hatching equivalent to viability. The experiment was done in duplicate with separate broods of cysts and the error bars represent standard error.