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. 2012 Aug 27;7(8):e43958. doi: 10.1371/journal.pone.0043958

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© 2012 Mishra et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Phospholipid spots shown in 2D-TLC plates were developed with CuSO4 (100 mg/ml) spray containing 8% phosphoric acid and heated at 180°C. ACP-1 and ACP-2, amino-containing PL-1 and -2; I-LPG, inner CM layer-lysyl-phosphatidylglycerol; O-LPG, outer CM layer lysyl-phosphatidylglycerol; PG, phosphatidylglycerol; CL,cardiolipin; GP-DGDAG, glycerolphospho-diglycodiacylglycerol; PA, phosphatidic acid; Un, unidentified phospholipid. Red arrows indicate the major spots confirmed by LC/ESI-MS/MS analysis. The spot of fluorescamine-labelled O-LPG was detected by UV, indicating flipping of the latter PL to the outer CM.