Figure 2. Thermostability of CbCelA-TM1 in presence of CbHsp18 (A), MkHistone1 (B), and RNase A (C) and hydrolysis of Avicel with CbCelA-TM1 in presence of CbHsp18 (D), MkHistone1 (E), and RNase A (F).

For the thermostability assay, 1 µM of CbCelA-TM1 was incubated with 8 µM, 16 µM, or 32 µM of CbHsp18, MkHistone1, or RNase A in a pH 6.0 citrate buffer. The mixtures were shaken end-over-end at 70°C for 24 hr. As a control, 1 µM of CbCelA-TM1 in the same buffer was incubated without shaking at 4°C. For hydrolysis of Avicel, 1 µM of CbCelA-TM1 was incubated with 20 mg/ml Avicel in the absence or presence of 8 µM, 16 µM, or 32 µM of CbHsp18, MkHistone1, or RNase A in a pH 6.0 citrate buffer. The reaction mixtures were shaken end-over-end at 70°C for 24 hr.