Table 2. End products of hydrolysis of Avicel by CbCelA-TM1/CbCdx1A in the presence or absence of non-GH proteinsa.
Glycoside hydrolases | Non-GH protein | Protein concentration (µM) | Glucose (mM) |
CbCelA-TM1+CbCdx1A | n/a | n/a | 7.4±0.5 |
CbHsp18 | 16 | 10.9±0.7 | |
32 | 11.1±0.5 | ||
64 | 10.8±0.5 | ||
MkHistone1 | 16 | 10.8±0.0 | |
32 | 10.8±0.3 | ||
64 | 11.0±0.0 | ||
RNase A | 16 | 12.1±0.1 | |
32 | 12.1±0.1 | ||
64 | 12.1±0.3 |
The hydrolysis was carried out at 70°C for 24 hr under an end-over-end shaking condition. The hydrolysis products after 24 hr were analyzed by HPLC. GH: glycoside hydrolase.