Table 3. End products of hydrolysis of Miscanthus by CbXyn10A in the presence or absence of non-GH proteinsa.
Glycoside hydrolase | Non-GH protein | Protein concentration (µM) | Xylose (mM) | Xylobiose (mM) | Xylotriose (mM) |
CbXyn10A | n/a | n/a | 0.4±0.2 | 1.5±0.0 | 0.3±0.0 |
CbHsp18 | 8 | 1.8±0.1 | 2.0±0.1 | 0.3±0.2 | |
16 | 2.1±0.1 | 2.1±0.0 | 0.2±0.0 | ||
32 | 2.6±0.0 | 2.3±0.0 | 0.4±0.1 | ||
MkHistone1 | 8 | 1.9±0.2 | 2.0±0.2 | 0.3±0.0 | |
16 | 2.2±0.2 | 2.1±0.1 | 0.3±0.0 | ||
32 | 2.8±0.1 | 2.5±0.0 | 0.1±0.0 | ||
RNase A | 8 | 1.2±0.1 | 1.8±0.0 | 0.2±0.0 | |
16 | 1.6±0.0 | 2.0±0.0 | 0.2±0.0 | ||
32 | 2.3±0.2 | 2.3±0.1 | 0.1±0.0 |
The hydrolysis was carried out at 70°C for 24 hr in an end-over-end shaking condition. The hydrolysis products after 24 hr were analyzed by HPLC. GH: glycoside hydrolase.