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. 2012 Aug 27;7(8):e43828. doi: 10.1371/journal.pone.0043828

Table 3. End products of hydrolysis of Miscanthus by CbXyn10A in the presence or absence of non-GH proteinsa.

Glycoside hydrolase Non-GH protein Protein concentration (µM) Xylose (mM) Xylobiose (mM) Xylotriose (mM)
CbXyn10A n/a n/a 0.4±0.2 1.5±0.0 0.3±0.0
CbHsp18 8 1.8±0.1 2.0±0.1 0.3±0.2
16 2.1±0.1 2.1±0.0 0.2±0.0
32 2.6±0.0 2.3±0.0 0.4±0.1
MkHistone1 8 1.9±0.2 2.0±0.2 0.3±0.0
16 2.2±0.2 2.1±0.1 0.3±0.0
32 2.8±0.1 2.5±0.0 0.1±0.0
RNase A 8 1.2±0.1 1.8±0.0 0.2±0.0
16 1.6±0.0 2.0±0.0 0.2±0.0
32 2.3±0.2 2.3±0.1 0.1±0.0
a

The hydrolysis was carried out at 70°C for 24 hr in an end-over-end shaking condition. The hydrolysis products after 24 hr were analyzed by HPLC. GH: glycoside hydrolase.