Figure 6. Effect of CDF and/or anti-miR-21 on miRNA expression and miR-21-mediated luciferase activity in human PCa cells under hypoxic conditions.

The panels A and B represent the data of miRNAs in the CSC-like sphere-forming cells derived from human PCa cells and luciferase activities in PCa cells, respectively. The transfection of miR-21-mediated reporter gene luciferase vector and anti-miR-21 were conducted in PC-3 cells, as described in detail under the Methods section. The luciferase activity was measured by using Promega luciferase assay system kit, following the manufacturer’s manual. The bars in the figures indicate standard deviation of n = 3.