Figure 5. Modulation of Dectin-1 expression by N10K.

Monocytes (1×10⁶/ml) were incubated for 30 min in the presence/absence (NS) of zymosan (100 µg/ml), NC, N10K, H4L, S8K, G7R or T11F (all 10 µg/ml) (A). In selected experiments, monocytes (1×10⁶/ml) were pre-treated for 30 min in the presence/absence (NS) of NC, N10K, H4L, S8K, G7R or T11F (all 10 µg/ml) and subsequently stimulated for 3 h with zymosan (50 µg/ml) (B). After incubation, cells were labelled with RPE mAb to Dectin-1 and analyzed by FACScan flow cytometry. MFI of labelled cells is shown as a histogram or as a FACScan histogram (A and B). *, P<0.05 (n = 7; treated vs untreated cells). Error bars denote s.e.m.