Figure 8. Proinflammatory cytokine production.

Monocytes (5×10⁶/ml) were pre-treated in the presence/absence (NS) of NC, N10K (both 10 µg/ml) or piceatannol (Pic) (100 µM) for 30 min and subsequently incubated with heat inactivated C. albicans cells (HI CA-6, E/T:1/10), curdlan (25 µg/ml) or zymosan (50 µg/ml) for 18 h. After incubation, cytokine levels were evaluated in culture supernatants using specific ELISA assays. (A) *, P<0.05 (n = 5; N10K treated vs untreated cells). Error bars denote s.e.m. In selected experiments monocytes (5×10⁶/ml) were treated in the presence/absence (NS) of NC, N10K (both 10 µg/ml) or zymosan (100 µg/ml) for 6 h, 18 h or 48 h. After incubation, cytokine levels were evaluated in culture supernatants using specific ELISA assays. (B) *, P<0.05 (n = 5; treated vs untreated cells).