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. 2012 Aug 27;7(8):e43577. doi: 10.1371/journal.pone.0043577

Figure 6. TGF treatment of AKT overexpression overrides the effects of PEITC.

Figure 6

(A) Representative blot of time dependent effect of 15 µM PEITC on p-EGFR (Tyr 1068), p-AKT (Ser-473), Cl-PARP in SKOV-3 ovarian cancer cells. Actin was used as loading control. (B) OVCAR-3 cells were stimulated with 50 ng/mL TGF for 1 hour after treatment with varying concentrations of PEITC for 24 hours. Whole-cell lysates were resolved on 10% SDS-PAGE for the analysis of phosphorylation of EGFR at Tyr-1068, phosphorylation of AKT at Ser-473, and cleavage of caspase-3 and PARP. Actin was used as a control for loading. Effect of AKT overexpression on PEITC induced apoptosis was also determined in SKOV-3 cells. (C) Representative blots showing p-AKT (Ser 473), AKT and Cl-PARP. Actin was used as a loading control.