Table 3. Variants of the glycosyltransferases involved in synthesis of LOS outer core structures in C. jejuni strains BD-07, BD-10, BD-39 and BD-67a.
Strains | LOS class | GeneBank Accession No. | Cj1135 | Cj1136 | CgtAI | CgtAII | CgtB | Cst-II | GalTg |
BD-07 | A | GU289927 | One-domain | Onb | Mono-sialyl.c | Absent | Mono-sialyl. | Mono-f | Absent |
BD-10 | B | GQ249164 | Two-domain | On | Non-sialyl.d | Offe | Off | Bi- | Present |
BD-39 | A | GU289928 | One-domain | On | Mono-sialyl. | Absent | Mono-sialyl. | Mono- | Absent |
BD-67 | B | GQ249165 | Two-domain | On | Non-sialyl. | Off | Off | Bi- | Present |
Assignment of the glycosyltransferase variants is based on amino acid sequence comparisons with variants of known specificities.
on: indicates that a gene has no frame-shift mutation.
Mono-sialyl.: the glycosyltransferase is specific for mono-sialylated acceptors.
Non-sialyl.: the glycosyltransferase is specific for non-sialylated acceptors.
off: indicates that a gene is inactive because of a frame-shift mutation.
Cst-II variants: mono-: monofunctional, Cst-II has α-2,3-sialyltransferase activity. bi-: bifunctional, Cst-II has both α-2,3-sialyltransferase and α-2,8-sialyltransferase activity.
GalT: β-1,4-galactosyltransferase that uses Glcβ-1,2-HepII- as acceptor.