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. 2012 Aug 27;7(8):e44053. doi: 10.1371/journal.pone.0044053

Table 3. Primer sequences used in real-time PCR.

Gene Sequence NCBI ID
LDLR Forward 5′-GAA CTC AGG GCC TCT GTC TG-3′ NM_010700
Reverse 5′-AGC AGG CTG GAT GTC TCT GT-3′
SREBP1 Forward 5′-GTG AGC CTG ACA AGC AAT CA-3′ NM_011480
Reverse 5′-GGT GCC TAC AGA GCA AGA GG-3′
SREBP1a Forward 5′-CGG CTC TGG AAC AGA CAC TG-3′ NM_011480
Reverse 5′-AAG TCA CTG TCT TGG TTG TTG-3′
SREBP1c Forward 5′-ATC GGC GCG GAA GCT GTC GG-3′ NM_011480
Reverse 5′-AAG TCA CTG TCT TGG TTG TTG-3′
SREBP2 Forward 5′-GTG GAG CAG TCT CAA CGT CA-3′ NM_033218
Reverse 5′-TGG TAG GTC TCA CCC AGG AG-3′
SCAP Forward 5′-GAT GTG TTC CGG TCA CCT CT-3′ NM_001103162
Reverse 5′-TTG GTC CCT GAG CTGTCT CT-3′
INSIG-1 Forward 5′-ACA CGTGGG ACC TAA CTT GC-3′ NM_153526
Reverse 5′-CTT CTC CGG AAT AGC TCG TG-3′
S1P Forward 5′-TCC CCA GCA GAG ACA GAG TT-3′ NM_019709
Reverse 5′-GTG GTA CTG GTC CCA GAG GA-3′
S2P Forward 5′-CAC TGG GAC TCT GGA TGG TT-3′ NM_172307
Reverse 5′-TTG GCC AAG GTG TTT TAA GG-3′
PER1 Forward 5′-GTG TCG TGA TTA AAT TAG TCA G-3′ NM_011065
Reverse 5′-ACC ACT CAT GTC GTC TGG GCC-3′