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. 2012 Aug 27;7(8):e41644. doi: 10.1371/journal.pone.0041644

Figure 1. Constitutive expression of canonical NF-κB signaling in SLE patients.

Figure 1

Isolated primary B cells were lysed and whole cell extractions were generated and then examined by western blot. Canonical NF-κB signaling was shown by phosphorylation and degradation of IκBα, phosphorylation of P65 (Fig. 1A); as well as nuclear translocation of P65, P50, c-Rel, Rel-B and P52 (Fig. 1B) (Y axis represents absorbance at 655 nm). Fig. 1A showed the results of three blots performed on three different controls and three different lupus patients. In Fig. 1B, data are depicted as the mean value± SD of ten independent experiments performed on ten different controls and ten different lupus patients. Statistical significance is determined by the one-tailed paired two-sample Student's t test and is indicated as follows: *p≤0.05).