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. 2012 Aug 27;7(8):e41644. doi: 10.1371/journal.pone.0041644

Figure 4. Effects of antagonistic anti-CD154 on nuclear translocation of P50, P65 and c-Rel as well as phosphorylation of P65 and IκBα in B cells from active lupus patients and tonsil B cells.

Figure 4

B cells from active, non-active lupus patients and tonsil B cells were incubated in the presence or absence of 10 µg/ml TRAP1 (anti-CD154) for 2 hours. Nuclear proteins were isolated and assessed for NF-κB binding activity of P50, RelA/P65, and c-Rel by NF-κB transfactor assays (Fig. 4A). Y axis represents absorbance at 655 nm. Data are depicted as the mean value± SD. Statistical significance is determined by the one-tailed paired two-sample Student's t test and is indicated as follows: *p≤0.05. Whole cell lysate was tested for phosphorylation of P65 and IκBα in B cells from active SLE patients by western blot (Fig. 4B). Results from one of five independent experiments are shown.