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. 2012 Aug 27;7(8):e43610. doi: 10.1371/journal.pone.0043610

Figure 5. NF-κB activation enhances hTREX84 expression in immortal and/or cancer cells.

Figure 5

A, Schematic diagram of the hTREX84 promoter indicating the conserved NF-κB DNA binding motif. B, ChIP assay of RelA/p65 binding to hTREX84 gene promoter in MDA-MB-231 (lane 1, 2); OVCAR5 (lane 3, 4); OVCAR 10 (lane 5, 6). Cells were cultured for 48 h. ChIP assays were then performed with anti-RelA/p65 antibody. PCR analysis was performed on immunoprecipitation samples without antibody (lane 1, 3, 5), with RelA/p65 antibody (lane 2, 4, 6). C, MCF-10F cells were transiently transfected with a control vector (lane 1) or a RelA/p65 cDNA expression construct for 48 hours. Western blot analysis for RelA/p65, hTREX84 and β-actin. D, Western blot analysis of RelA/p65, hTREX84 and β-actin protein levels after treatment of MDA-MB-231 cells with control siRNA (lane 1) and siRNA against RelA/p65 (lane 2) for 72 hours.