Figure 3. Methylation profiles of spermatogenic cell-specific intronless genes are classified three groups in juvenile mouse testis.

COBRA was carried out to examine the methylation status of the selected genes, the CpG island in the Oaz1 gene, and constitutively methylated endogenous IAP retroviruses in genomic DNA isolated from D7, D14, D21, and D35 testes and adult liver. Sodium bisulfite-treated DNA was amplified with specific primers (details in Table S3) and digested with HpyCH4 IV (recognition site: ACGT), Taq I (TCGA) or Acc II (CGCG).