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. 2012 Aug 27;7(8):e43658. doi: 10.1371/journal.pone.0043658

Figure 4. Methylation profiles of spermatogenic cell-specific intronless genes are classified three groups in male germ cells.

Figure 4

COBRA was carried out to examine the methylation status of the selected genes, the CpG island in the Oaz1 gene, and constitutively methylated IAP in genomic DNA isolated from 15.5 dpc embryonic (E15.5) and neonatal (D0–1) gonocytes, spermatogonia (SG), pachytene spermatocytes (PS), round spermatids (RS), epididymal spermatozoa (SZ), and adult liver. The sodium bisulfite reaction and restriction enzyme digestion were carried out as described in Figure 3. The COBRA results are also represented by bar graphs.