Fig 1.

Pneumococcal loads in the lungs and blood of mice after challenge with the ATCC 6303 strain. Lungs (A) or blood (B) from nonimmunized BALB/c mice (Non) or mice immunized with wP or PspA5-wP were collected at different time points after the intranasal challenge. CFU were determined in four mice per group after plating the samples in blood agar. Circles represent each animal, and lines represent the mean for each group. # and *, significantly lower numbers of cells compared with those at the same time point in nonimmunized mice or mice immunized with wP, respectively. In panel A, P = 0.03 for all time points except at 36 h, when no significant difference was observed when mice immunized with PspA5-wP were compared with mice inoculated with wP. In panel B, P = 0.02 for the time points shown in the graph.