Fig 2.

Infiltration of cells in the respiratory tracts of mice after challenge with the ATCC 6303 strain. BALF samples were collected from nonimmunized BALB/c mice (Non) or mice immunized with wP or PspA5-wP at different time points after the intranasal challenge, and slides were prepared with 4 × 10⁴ cells. Numbers of total infiltrated cells (A), lymphocytes (B), neutrophils (C), or macrophages (D) are expressed as means for 4 mice per group with standard deviations. *, significantly higher numbers of cells compared with those at the same time point in nonimmunized mice; ○, significantly higher numbers of cells compared with those at the same time point in mice immunized with wP; #, significantly lower numbers of cells compared with those at the same time point in nonimmunized mice; ●, significantly lower numbers of cells compared with those at the same time point in mice immunized with wP. Statistical analyses were performed with the Mann-Whiney U test, and P ≤ 0.05 for all the comparisons marked.