Fig 9.
Absence of antibody binding to the pneumococcal surface of 6303PspA− mutants. BALF samples from mice immunized with PspA5-wP were collected 21 days after the last immunization, and pools (from 3 mice) were incubated with different mutants derived from the ATCC 6303 strain or the wild-type strain. BALF from nonimmunized mice was used as control. Samples were incubated with FITC-conjugated anti-mouse IgA or IgG, and the results were analyzed by flow cytometry with 10,000 events gated. The median fluorescence intensity is indicated for each sample. Data are representative of two experiments using different pools.