Table 1.
Gene | Primer direction | Primer sequencea | Product size (bp) (amplified region [nt positions])b |
---|---|---|---|
tsa56 | Forward | GGCGGATCCCCAGGATTTAGAGCAGAG | 1,344 (253–1596) |
Reverse | CGGTCGACGAAGTTATAGCGCACA | ||
p47 | Forward | CGGGATCCATTAGTGTAAATAGTTTATCC | 1,278 (121–1398) |
Reverse | CGGTCGACCTTATTAATATTAGGTAAAGC | ||
scaA | Forward | CGGGATCCTCATCTGCTAGAGGTGAGATG | 1,169 (2371–3540) |
Reverse | CGGTCGACATCGCCTTTTAAACCCGGGTTACT | ||
Forward | GGCGGATCCTTACTTGGAGATTATACTG | 1,121 (2414–3534) | |
Reverse | CGCTCGAGTTGTTTATTGATATTAGATCC | ||
scaB | Forward | GGCGGATCCAGTACAACTCAAAGGATATTAGG | 1,047 (70–1116) |
Reverse | CGGTCGACACTACTACAAATGTTTGATCC | ||
scaC | Forward | GGCGGATCCAAAAGTATAACTCCAGAAAAGTG | 600 (97–696) |
Reverse | CGGTCGACGTTTAATTTAGCACGATTTAT | ||
Forward | GGCGGATCCATGTACCAATCTAAT | 1,578 (1–1578) | |
Reverse | CGGTCGACAAAATTAGTTCCTATATG | ||
scaD | Forward | AAGGATCCCAATTAAGTGAGCGACTA | 1,926 (136–2061) |
Reverse | CGGAATTCTACCGTAGCAACAGTAACAGC | ||
scaE | Forward | GGCGGATCCAATGTAAATGCACAGCCCAATAG | 1,332 (91–1422) |
Reverse | CGGTCGACCTGTACTGTTGCTATTTTAGA | ||
Forward | GGCGGATCCAATGTAAATGCACAGCCCAA | 1,291 (111–1401) | |
Reverse | CGGTCGACCTGTACTGTTGCTATTTTAGA |
Restriction enzyme sites are underlined.
For Boryong strain genes.