Table 1.
Primer sequences used in this study
| Gene | Primer direction | Primer sequencea | Product size (bp) (amplified region [nt positions])b |
|---|---|---|---|
| tsa56 | Forward | GGCGGATCCCCAGGATTTAGAGCAGAG | 1,344 (253–1596) |
| Reverse | CGGTCGACGAAGTTATAGCGCACA | ||
| p47 | Forward | CGGGATCCATTAGTGTAAATAGTTTATCC | 1,278 (121–1398) |
| Reverse | CGGTCGACCTTATTAATATTAGGTAAAGC | ||
| scaA | Forward | CGGGATCCTCATCTGCTAGAGGTGAGATG | 1,169 (2371–3540) |
| Reverse | CGGTCGACATCGCCTTTTAAACCCGGGTTACT | ||
| Forward | GGCGGATCCTTACTTGGAGATTATACTG | 1,121 (2414–3534) | |
| Reverse | CGCTCGAGTTGTTTATTGATATTAGATCC | ||
| scaB | Forward | GGCGGATCCAGTACAACTCAAAGGATATTAGG | 1,047 (70–1116) |
| Reverse | CGGTCGACACTACTACAAATGTTTGATCC | ||
| scaC | Forward | GGCGGATCCAAAAGTATAACTCCAGAAAAGTG | 600 (97–696) |
| Reverse | CGGTCGACGTTTAATTTAGCACGATTTAT | ||
| Forward | GGCGGATCCATGTACCAATCTAAT | 1,578 (1–1578) | |
| Reverse | CGGTCGACAAAATTAGTTCCTATATG | ||
| scaD | Forward | AAGGATCCCAATTAAGTGAGCGACTA | 1,926 (136–2061) |
| Reverse | CGGAATTCTACCGTAGCAACAGTAACAGC | ||
| scaE | Forward | GGCGGATCCAATGTAAATGCACAGCCCAATAG | 1,332 (91–1422) |
| Reverse | CGGTCGACCTGTACTGTTGCTATTTTAGA | ||
| Forward | GGCGGATCCAATGTAAATGCACAGCCCAA | 1,291 (111–1401) | |
| Reverse | CGGTCGACCTGTACTGTTGCTATTTTAGA |
a
Restriction enzyme sites are underlined.
b
For Boryong strain genes.