Fig 2.

Membrane-associated antigen identified by detergent extraction. A postsonication particulate fraction from B. microti-infected hamster red blood cells was extracted using Triton X-114 detergent as described in Materials and Methods. Extracted proteins were resolved on a 10 to 22.5% polyacrylamide gel and transferred to PVDF. Strips of membrane were incubated with sera from an infected hamster (A), sera from a babesiosis-positive patient (B), or AuroDye Forte colloidal gold stain (C). Bound IgG antibodies were visualized as described for Fig. 1. The positions of molecular mass markers (in thousands) are indicated on the left. A major immunodominant band is indicated by an arrow just above the 36-kDa marker.