Table 3.
Comparison of double-antigen sandwich ELISA with IIF and commercial indirect ELISA kit
| Double-antigen sandwich ELISA resulta | No. of serum samples |
|||||
|---|---|---|---|---|---|---|
| IIF |
Commercial kitb |
|||||
| Positive | Negative | Total | Positive | Negative | Total | |
| Positive | 251 | 7 | 258 | 252 | 6 | 258 |
| Negative | 26 | 110 | 136 | 15 | 121 | 136 |
| Total | 277 | 117 | 394 | 267 | 127 | 394 |
S/P ratio cutoffs: positive, ≥0.27; negative, <0.27.
For better statistical analysis, the S/P ratio cutoffs of the commercial kit were adjusted as follows: positive, ≥0.35; negative, <0.35 (the original S/P ratio cutoffs were positive, ≥0.40; equivocal, ≥0.30 and <0.40; negative, <0.30).