Table 6.
Cytokine and dectin mRNA expression in MLN, IPP, and ASC of nonvaccinated and influenza vaccine-treated 21-day-old pigletsa
| Sample and cytokine or dectin | mRNA expression |
Model P valueb | |||
|---|---|---|---|---|---|
| NV |
V |
||||
| WGP0 | WGP90 | WGP0 | WGP90 | ||
| MLN | |||||
| IL-2 | 1.0 ± 0.7 | 0.5 ± 0.5 | 1.3 ± 0.3 | 0.5 ± 0.4 | 0.1601 |
| IL-12 | 1.0 ± 0.6 | 0.5 ± 0.4 | 0.4 ± 0.4 | 0.7 ± 0.3 | 0.4369 |
| IL-4 | 1.0 ± 0.4 | 0.6 ± 0.3 | 1.1 ± 0.3 | 1.1 ± 0.3 | 0.4978 |
| Dectin | 1.0 ± 0.3 | 0.6 ± 0.5 | 0.8 ± 0.5 | 0.9 ± 0.3 | 0.6668 |
| IPP | |||||
| IL-2 | 1.0 ± 0.6 | 0.8 ± 0.3 | 1.2 ± 0.4 | 3.6 ± 0.2 | 0.3157 |
| IL-12 | 1.0 ± 0.3 | 0.8 ± 0.2 | 1.0 ± 0.6 | 1.5 ± 0.2 | 0.7665 |
| IL-4 | 1.0 ± 0.3 | 1.4 ± 0.1 | 1.6 ± 0.2 | 1.0 ± 0.2 | 0.1392 |
| Dectin | 1.0 ± 0.2 | 2.5 ± 0.2 | 2.0 ± 0.1 | 1.2 ± 0.1 | 0.4426 |
| ASC | |||||
| IL-2 | 1.0 ± 0.2 | 0.8 ± 0.7 | 0.6 ± 2.0 | 1.0 ± 0.2 | 0.5407 |
| IL-12 | 1.0 ± 0.4 | 0.9 ± 0.4 | 0.4 ± 0.8 | 1.1 ± 1.3 | 0.7454 |
| IL-4 | 1.0 ± 0.3 | 0.2 ± 1.0 | 0.3 ± 0.8 | 1.1 ± 0.9 | 0.9978 |
| Dectin | 1.0 ± 1.1 | 0.4 ± 0.5 | 0.2 ± 0.3 | 0.3 ± 0.2 | 0.4514 |
a
Data are expressed as means ± SD relative to fold difference of gene expression in nonvaccinated, formula diet samples at day 21. NV, nonvaccinated; V, vaccinated.
b
Model included diet and vaccination. Interaction between diet and vaccination was previously run and was found to be nonsignificant. All diet and vaccination P values > 0.05.