Table 7.
Cytokine and dectin mRNA expression in spleen of nonvaccinated and human influenza vaccine-treated 21-day-old pigletsa
| Cytokine or dectin | mRNA expression |
Model P valueb (vaccination) | |||
|---|---|---|---|---|---|
| NV |
V |
||||
| WGP0 | WGP90 | WGP0 | WGP90 | ||
| IL-2 | 1.0 ± 1.1 | 1.3 ± 1.1 | 12.4 ± 5.9 | 2.5 ± 2.4 | 0.0321 (0.0056) |
| IL-12 | 1.0 ± 1.1 | 13.8 ± 4.7 | 6.3 ± 3.9 | 2.8 ± 1.4 | 0.4981 (0.6394) |
| IL-4 | 1.0 ± 0.5 | 0.6 ± 0.4 | 3.8 ± 1.5 | 1.3 ± 0.7 | 0.0004 (<0.0001) |
| IL-6 | 1.0 ± 0.5 | 1.0 ± 0.4 | 3.1 ± 1.1 | 2.2 ± 1.6 | 0.0299 (0.001) |
| TNF-α | 1.0 ± 0.3 | 3.3 ± 2.7 | 5.3 ± 1.5 | 2.1 ± 1.7 | 0.1388 (0.0451) |
| Dectin | 1.0 ± 0.6 | 0.6 ± 0.4 | 4.3 ± 2.8 | 1.4 ± 0.6 | 0.0226 (0.0046) |
| TGF-β2 | 1.0 ± 0.3 | 1.9 ± 2.7 | 6.4 ± 4.5 | 1.6 ± 1.0 | 0.0500 (0.084) |
a
Data are expressed as mean ± SD, relative to fold difference of gene expression in day 21 nonvaccinated formula diet group. NV, nonvaccinated; V, vaccinated.
b
Model included diet, vaccination, and interaction: model P value (vaccination P value). All diets and interaction were nonsignificant (P > 0.05).