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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: Gen Comp Endocrinol. 2012 Jul 25;178(3):562–572. doi: 10.1016/j.ygcen.2012.07.011

Figure 4. Rescuing leptin morphant defects using recombinant leptin.

Figure 4

Panels A-D show lateral views (anterior to the left and dorsal up) of live zebrafish embryos at 49 hpf. A rescued embryo (panel B) appears to be similar in gross morphology to an uninjected control embryo (panel A). Panels E-H show embryos processed for whole mount immunostaining using zn5 antibody. Panels E and F show ventral views (anterior to the top) of the head region while panels G and H show lateral views (anterior to the left) of the mid-trunk region. Abbreviations: 1:1 LepRes, 1 part recombinant leptin: 1 part lepMO1; 1:4 LepRes, 1 part recombinant leptin: 4 parts lepMO1; gcl, retinal ganglion cell layer; on, optic nerve; te, telencephalon; scd, spinal cord; sn, spinal nerves; ye, yolk extension. Other abbreviations are the same as in Figure 3. Scale bars = 250 μm for panels A-D, and 50 μm for panels E-H.