. Author manuscript; available in PMC: 2013 Sep 15.

Published in final edited form as: Gen Comp Endocrinol. 2012 Jul 25;178(3):562–572. doi: 10.1016/j.ygcen.2012.07.011

Table 2.

Effects of Recombinant Leptin Rescue on Zebrafish Development

	# embryos with slight gross defects (%)	# embryos with moderate gross defects (%)	# embryos with severe gross defects (%)	# embryos examined at 48-50 hpf (# embryos with wild type phenotype)
Uninjected Control	0 (0.0%)	1 (2.0%)	0 (0.0%)	49 (98%)
lepMO1 (0.5 mM, 2 nl)	3 (7.7%)	20 (51.3%)	16 (41.0%)	0 (0%)
lepMO1 (0.5 mM, 2 nl) + 1:25 zleptin (40 nM, 2nl)	1 (2.3%)	25 (56.8%)	18 (40.9%)	0 (0%)
lepMO1 (0.5 mM, 2 nl) + 1:4 zleptin (7.5 μM, 2nl)	5 (14.7%)	28 (82.3%)	1 (3.0%)	0 (0%)
lepMO1 (0.5 mM, 2 nl) + 1:1 zleptin (30 μM, 2nl)	48 (70.6%)	19 (27.9%)	1 (1.5%)	0 (0%)