Table 3.
Effects of LepMO or LepRMO Injections on Expression of Neural Markers
| forebrain and eye (%) | midbrain (%) | bmh (%) | cerebellum (%) | hindbrain (%) | spinal cord (%) | spinal nerves | |
|---|---|---|---|---|---|---|---|
| 25 hpf krox20 | |||||||
| Control (n=10) | 0 | ||||||
| LepMO1 (n=10) | 0 | ||||||
| 25 hpf pax2a & shh | |||||||
| Control (n=10) | 10.0 | 0 | 0 | ||||
| LepMO1 (n=9) | 0 | 11.0 | 0 | ||||
| 49 hpf anti-Hu | |||||||
| Control (n=26) | 7.7 | 7.7 | 0 | 3.8 | |||
| 5-misMO (n=12) | 16.7 | 8.3 | 8.3 | 0 | |||
| LepMO1 (n=15) | 100 | 100 | 100 | 100 | |||
| LepRMO (n=8) | 100 | 100 | 100 | 100 | |||
| 49 hpf zn5 | |||||||
| Control (n=25) | 0 | 8.0 | 12.0 | 12.0 | |||
| 5-misMO (n=15) | 0 | 13.3 | 20.0 | 12.0 | |||
| LepMO (n=15) | 100 | 13.3 | 40.0 | 100 | |||
| LepRMO (n=12) | 100 | 25.0 | 33.3 | 100 | |||
| 49 hpf neuroD | |||||||
| Control (n=10) | 0 | 0 | 0 | 0 | |||
| LepMO (n=10) | 100 | 100 | 100 | 100 |
n, number of embryos examined. %, percentages of obviously abnormally staining (e.g. reduced staining intensity or region within the structure compared to the majority of control embryos).