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. Author manuscript; available in PMC: 2013 Aug 24.
Published in final edited form as: Immunity. 2012 Aug 2;37(2):290–301. doi: 10.1016/j.immuni.2012.05.021

Figure 7. Circadian time influences leukocyte recruitment in septic shock.

Figure 7

(A) Bioluminescence imaging and quantifications of harvested liver after adoptive transfer of Vav-Cre:luciferase-expressing BM cells to recipients at ZT5 or ZT13. n = 7. (B–C) Quantification of endothelial cell adhesion molecule expression levels in liver at steady state (B) and after 12h stimulation with LPS (C) by immunofluorescence confocal imaging of frozen tissue sections. n = 4–7. (D) Quantification of LPS-induced neutrophil infiltration in frozen liver sections of WT or Icam1−/− animals. n = 6. (E–F) Representative images of neutrophil infiltration in liver sections as assessed by immunofluorescence confocal imaging (E) and H&E staining (F) showing neutrophils clusters (arrows). (G–H) Survival curves of WT (G) and Icam1−/− (H) animals after LPS-induced septic shock. n = 7–17. *P<0.05, **P<0.01. Scale bars: 50 µm. See also Figure S7.