Fig. 3.
I3C and DIM do not directly inhibit HDAC activity. Nuclear extracts containing HDAC enzymes were incubated with increasing concentrations of I3C or DIM. The vehicle (DMSO), 20 nM TSA, and no treatment controls were also included. Data represent an average of 3 independent measurements ± SEM. *** indicates statistical significance of p<0.001 compared to DMSO control samples.