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Effect of DIM on class II HDACs. LNCaP and PC-3 cells were treated with increasing concentrations of DIM or DMSO for 48 hours. Data are densitometry results of Western blots for A) HDAC4 and B) HDAC6 protein levels normalized to [g533]-actin and the DMSO control. Data are expressed as a percentage of the DMSO control treatment and represent an average of 3-7 independent experiments ± SEM. No significance difference was detected with DIM treatment.
