Fig. 7.
HDAC2 knockdown in LNCaP cells results in increases in acetylated histone levels. LNCaP cells were transfected with HDAC2 siRNA, control scrambled siRNA, or vehicle control. A) HDAC2 mRNA expression was evaluated 48h following transfection. B) HDAC1 and HDAC2 protein expression was determined 72h following transfection. C) Acetylated histone H4 protein levels normalized to [g533]-actin and H4 protein levels 72 hours following HDAC2 siRNA knockdown. Statistical significance as compared to scrambled control siRNA: *p<0.05, ***p<0.001.