Fig. 4.

Expression of microRNAs in GFAP-positive astrocytes in mTLE. To characterize the small-sized cells expressing miR-92b, miR-637 and miR-665 in the hippocampus of mTLE patients, miRNA-ISH was combined with immunohistochemistry for glial fibrillary acidic protein (GFAP) on hippocampal sections of mTLE−HS and mTLE + HS patients. The miRNA-ISH signal is pseudocolored in green, nuclear DAPI staining in blue and GFAP labeling in red. The lower part of each panel shows images of individual astrocytes at a higher magnification. In mTLE, but not control (not shown), patients miR-92b (a, b), miR-637 (c, d) and miR-665 (e, f) expression is predominantly localized to the nucleus of GFAP-positive astrocytes. Scale bar 40 μm