Fig. 3.

Subcellular localization of deletion mutants of AS2 in M phase cells of transformed BY-2 lines. BY-2 cells harboring each mutant construct were incubated for 16 h in the presence of 0.05 μM 17-β-estradiol. Cells were fixed and stained with DAPI. Fluorescence of DAPI (blue) and fluorescence of YFP (yellow) in the M-phase cells are visualized by using confocal fluorescence microscopy. Nomarski images (DIC) and merged images of YFP and DAPI (Merged) are shown in the left panels and the right panels, respectively. Numbers on the right represent ratios of cells showing AS2 bodies to total M phase cells examined. In anaphase and telophase cells, AS2 bodies that seem to belong to the same pair are marked with a common shape of arrowheads or arrows. Bars 20 μm