Figure 6. Effects of Celecoxib on the expression of TIMP1 by corneal fibroblasts.

Cells were cultured in collagen gels for 48 hours in the presence of plasminogen, in the absence or presence of IL-1ß(0.1ng/mL), and in the presence of the indicated concentrations of Celecoxib. The culture supernatants were then subjected to immunoblot analysis with antibodies to TIMP1. Data are representative of three independent experiments. The positions of bands corresponding to the TIMP1 are indicated on the right, and those of molecular size are shown on the left.