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. Author manuscript; available in PMC: 2013 Aug 24.
Published in final edited form as: Chem Biol. 2012 Aug 24;19(8):1049–1059. doi: 10.1016/j.chembiol.2012.07.004

Figure 3.

Figure 3

Activation of the aza pathway via overexpression of azaR. (A) Transcriptional analysis of the aza genes in (i) A. niger WT and (ii) the activated T1 strain by RT-PCR. (ii) PCR from genomic DNA are shown for comparison. # indicates intron-less genes. (B) Time course of A. niger T1 metabolites. Metabolic profiles of the activated A. niger T1 strain on day 2, 4 and 7; and WT profile at day 2 are shown (C) Compounds observed in culture of A. niger T1 strain (see Figure S1). Structures indicated with * are confirmed by NMR (see Tables S1-3 and Figures S6-10). MS/MS analyses of 3 and 4 are in Figures S3-4.