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Deletion of azaB confirms the involvement of the cluster in biosynthesis of 1. (A) Knockout strategy employed for azaB. Correct integration is verified by using 3 sets of primers shown. (B) HPLC traces demonstrating the abolishment of 1 production in the azaB strain and the accumulation of 6 and 7 (See Table 3 and Figures S9-10).
