Table 1.
Putative functions and homologs of the genes in the aza cluster.
| Locus Tag (ASPNIDRAFT_) |
Gene Name |
Putative function | Characterized Homolog |
Protein Identity / Similarity (%) |
|---|---|---|---|---|
| 54405 | n/a | hydrolase | - | - |
| 127101 | n/a | thioesterase | - | - |
| 50208 | azaC | esterase/lipase | AN1032.3, afoC | 44/56 |
| 189181 | azaD | acyltransferase | F. sporotrichioides Tri101 | 33/49 |
| 212676 | azaE | ketoreductase | S. cerevisiae GRE2 | 35/50 |
| 188806 | azaF | acyl:CoA ligase |
Physcomitrella patens 4CL2 |
35/53 |
| 189194 | azaG | FAD-dependent oxygenase |
AN1035.3, afoF | 37/64 |
| 188800 | azaH | salicylate monooxygenase |
AN1033.3, afoD | 41/58 |
| 43449 | azaI | cytochrome P450 | A. flavus CypX | 30/49 |
| 56946 | azaA | NR-PKS | AN1034.3, afoE | 44/60 |
| 132962 | azaR | Zn2Cys6 regulator | AN1029.3, afoA | 34/48 |
| 43447 | azaJ | dehydrogenase | AN1030.3 | 40/53 |
| 188912 | azaK | efflux transporter | - | - |
| 132654 | azaL | FAD-dependent oxygenase |
AN1035.3, afoF | 33/55 |
| 188817 | azaB | HR-PKS | AN1036.3, afoG | 43/60 |
| 188504 | n/a | cytochrome P450 | - | - |
| 50199 | n/a | oxidoreductase | - | - |
Note: Assignment of genes to aza cluster is based on transcriptional analysis on A. niger T1 (Figure 3A); n/a, not assigned (upstream and downstream genes).