Table 1.
Primary antisera used for immunohistochemistry
| Antibody (clone) | Host | Cells identified | Dilution | Source of antibody |
|---|---|---|---|---|
| B-fabp | Rabbit polyclonal | glial cells | 1:300 | kind gift of Thomas Müller, (Kurz et al., 1994) |
| CN | Mouse IgG1 | neurons | 1:10 | kind gift of Hideaki Tanaka, (Tanaka et al., 1990) |
| GFAP | Rabbit polyclonal | glial cells | 1:500 | DAKO |
| HNK-1 | mouse IgM | ENCCs | 1:80 | Neomarkers |
| Hu (clone: 16A11) | Mouse IgG2a | neurons | 1:100 | Invitrogen |
| L1CAM (8D9) | Mouse IgG1 | ENCCs | 1:5 | DSHB |
| N-cadherin (6B3) | Mouse IgG1 | ENCCs | 1:10 | DSHB |
| NCAM (4d) | Mouse IgG1 | neurons | 1:5 | DSHB |
| Neurofilament (4H6) | Mouse IgG | neurons | 1:10 | DSHB |
| p75 | Rabbit polyclonal | ENCCs | 1:10000 | kind gift of Louis Reichardt, (Weskamp and Reichardt, 1991) |
| Sox10 | Mouse IgG | ENCCs and glial cells | 1:50 | kind gift of Michael Wegner, (Schmidt et al., 2003) |
| Tuj1 (B1195) | Mouse IgG2a | neurons | 1:200 | Covance |