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Model for P-body assembly in yeast. First, P-body factors are recruited to the mRNA as complexes. Second, interactions between P-body proteins in the complexes lead to the formation of a “closed-loop” structure. Finally, mRNPs aggregate via the Q/N domain of Lsm4, the Yjef-N domain of Edc3, or the amino-terminal domain of Pat1 to form microscopically visible cytoplasmic granules.
