Figure 5.

Dual staining of a human pancreatic cancer section for α smooth muscle actin (αSMA) and mRNA for collagen. The Figure depicts low and high power views of a pancreatic cancer section immunostained for αSMA and for collagen mRNA using in situ hybridisation. The brown staining for αSMA is co-localized with the blue staining for collagen mRNA. Importantly, both stains are restricted to the stromal areas of the section, with no staining of the tumour elements. The findings indicate that activated PSCs are the predominant source of collagen in the stroma of pancreatic cancer. Reprinted with permission from Wolters Kluwer Health.