Table 1.
Assay | Assay volume, μL | Substrate, mmol/L | Internal standard, nmol | Buffer | Detergent | Additional components |
---|---|---|---|---|---|---|
ABG | 25 | 0.2 | 0.2 | 0.37 mol/L citrate-phosphate, pH 5.1 | 9.6 g/L sodium taurocholate | None |
ASM | 25 | 0.2 | 0.1 | 0.55 mol/L sodium acetate, pH 5.5 | 0.6 g/L sodium taurocholate | None |
GALC | 25 | 0.2 | 0.1 | 0.18 mol/L citrate-phosphate, pH 4.5 | 9.6 g/L sodium taurocholate | 0.96 g/L oleate |
GAA | 25 | 0.4 | 0.1 | 0.18 mol/L citrate-phosphate, pH 4.0 | 0.6 g/L Triton X-100 | 80 μmol/L acarbose |
GLA | 12.5 | 2.0 | 0.1 | 0.34 mol/L sodium acetate, pH 4.6 | 1.2 g/L sodium taurocholate | 70 mmol/L N-acetylgalactosamine |