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Stereochemical determination by Marfey’s method. (A) (i) Structure of Nα-(2,4-dinitro-5-fluorophenyl)-l-valinamide (FDVA); (ii) derivatised diastereoisomers 29-1-FDVA and 29-2-FDVA; (iii) schematic representations of proposed cis- and trans-like diastereomer conformations.27 (B) Retention times of 32-1-FDVA and 32-2-FDVA and corresponding standard amino acids. The absorption peak at ∼18.50 corresponds to elution of unreacted FDVA.
